A new monoterpene glycoside from the roots of Paeonia lactiflora increases the differentiation of osteoblastic MC3T3-E1 cells.

نویسندگان

  • Pham Hai Yen
  • Phan Van Kiem
  • Nguyen Xuan Nhiem
  • Nguyen Huu Tung
  • Tran Hong Quang
  • Chau Van Minh
  • Jung Woo Kim
  • Eun Mi Choi
  • Young Ho Kim
چکیده

A new monoterpene glycoside, 6'-O-beta-D-glucopyranosylalbiflorin (1), and four known compounds; albiflorin (2), 6'-O-benzoylalbiflorin (3), paeoniflorin (4) and benzoyl paeoniflorin (5), were isolated from the methanolic extract of the roots of Paeonia lactiflora Pall.. Their chemical structures were completely elucidated using a combination of 2D NMR techniques (COSY, HMQC and HMBC) and HRESI-MS analyses. To investigate the bioactivities of these compounds, their effects on the differentiation of osteoblastic MC3T3-E1 cells were tested. Compound 1 (0.01-10 microM) significantly increased the alkaline phosphatase activity and nodules mineralization of MC3T3-E1 cells compared to those of the control (P<0.05). These results suggest that newly isolated compound 1 has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis.

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عنوان ژورنال:
  • Archives of pharmacal research

دوره 30 10  شماره 

صفحات  -

تاریخ انتشار 2007